Little Penguin is the smallest of the 18 penguin species in the world. Because they are at high risk of being preyed upon, they tend to hide on land, perhaps to protect themselves from foxes and dogs. For this reason, even at the aquarium, they often rest in the shade of rocks on the right side of the exhibit, except during feeding times. Some individuals rest very close to the barrier separating them from the viewing area, so be sure to observe them up close.

When feeding Little Penguin, the tray of fish is placed on the ground. It may seem a little strange to see penguins, which normally catch their food while swimming in the sea, picking up fish from a tray to eat. This is to prevent the powdered vitamins sprinkled on the fish from being washed away by the water. The fish given to Humboldt Penguin can have the vitamins placed in their mouths, but the fish for the fairy penguins are small, so the vitamins can only be sprinkled on them.

The breeding season for Little Penguin is from autumn to spring. In the wild, they build nests by digging holes in the ground or using crevices in rocks, but at the aquarium, they use caves carved into artificial rocks or wooden nest boxes. As the breeding season approaches, the keepers scatter rice straw near the nests as nesting material. If you're lucky, you might be able to see the penguins carrying the nesting material into the nest boxes.